{"id":4764,"date":"2024-09-19T15:59:34","date_gmt":"2024-09-19T15:59:34","guid":{"rendered":"https:\/\/ernestohernandez.org\/?p=4764"},"modified":"2024-09-20T10:58:47","modified_gmt":"2024-09-20T10:58:47","slug":"procedure-for-elemental-analysis-and-microscopy-sem-edx","status":"publish","type":"post","link":"https:\/\/ernestohernandez.org\/?p=4764","title":{"rendered":"Procedure for elemental analysis and microscopy (SEM-EDX)"},"content":{"rendered":"\n<p class=\"has-text-align-justify wp-block-paragraph\">BIERG offers open access to its Standard Operating Procedure (SOP) for simultaneous microscopy and elemental analysis. According to Ernesto Hernandez, the Principal Investigator, this technique is based on Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX). The procedure provides a step-by-step approach to microscopy and elemental analysis using the Hitachi TM4000 Plus SEM and Oxford Instrument Aztec ED Spectrometer (EDS). Under the right conditions, the procedure works well on various types of samples and it covers the analysis of materials from living systems such as biomass.<\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">The SOP details the process from sample preparation to final analysis. It includes guidance on the optimal size, shape, and preparation methods required to achieve accurate results. The user will be aware of the sources of interference as well as the importance of removing any moisture or contaminants.<\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">In this technique, SEM focuses a beam of electrons to interact with atoms in the sample, producing signals providing information about its morphology. In a second stage, the EDS emits X-rays, colliding with the sample, producing a fingerprint based on the nature and concertation of each particular element in the sample. The SOP guides users through setting the appropriate parameters in the AztecOne software for accurate identification of elements.<\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">This SOP is an extensive guide to SEM-EDX, offering a valuable resource for researchers across disciplines ranging from material science to biology to chemistry to engineering and more. The procedure ensures consistency, accuracy, and reliability in elemental analysis. This enhances the study of materials from living and non-living systems alike. <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Get a copy: <a href=\"https:\/\/doi.org\/10.5281\/zenodo.13736742\">https:\/\/doi.org\/10.5281\/zenodo.13736742<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>How to cite:<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Hernandez, J.E., Thomas, J.K. , Kheirandish, K., Wilson, C. and Ortega, H. (2024) Standard operating procedure for analysing of biomass through SEM-EDX. DOI: <a href=\"https:\/\/doi.org\/10.5281\/zenodo.13736742\">https:\/\/doi.org\/10.5281\/zenodo.13736742<\/a>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>BIERG released an SOP for using SEM-EDX microscopy and elemental analysis. It covers the Hitachi TM4000 Plus SEM and Oxford Instrument Aztec ED Spectrometer, advising on sample preparation, parameter settings, and contamination removal. The guide aids consistent, accurate analysis of materials from various fields, improving research reliability. Get it at https:\/\/doi.org\/10.5281\/zenodo.13736742.<\/p>\n","protected":false},"author":1,"featured_media":4769,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[101,12,111,105],"tags":[139,29,44,136],"class_list":["post-4764","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-analytical-chemistry","category-news","category-output","category-teaching","tag-microscopy","tag-research","tag-science-and-technology","tag-sem-edx"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/ernestohernandez.org\/wp-content\/uploads\/2024\/09\/Analysis-chemicals.png?fit=1080%2C1080&ssl=1","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p6rw5S-1eQ","jetpack-related-posts":[{"id":3887,"url":"https:\/\/ernestohernandez.org\/?p=3887","url_meta":{"origin":4764,"position":0},"title":"Job Biomass Analysis Research Internship","author":"Ernesto Hernandez","date":"January 5, 2023","format":"gallery","excerpt":"RoleCurrent climate change concerns and the post COVID world call for urgent solutions to develop sustainable societies. Nonfood biomass instead of petroleum is an option to produce cleaner biofuels, bioplastics and more.\u00a0 To achieve some sustainable goals, biomass needs to be transformed by sustainable manufacturing, which is in line with\u2026","rel":"","context":"In &quot;Jobs&quot;","block_context":{"text":"Jobs","link":"https:\/\/ernestohernandez.org\/?cat=124"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4083,"url":"https:\/\/ernestohernandez.org\/?p=4083","url_meta":{"origin":4764,"position":1},"title":"Job Research Internship BioRenewables","author":"Ernesto Hernandez","date":"February 5, 2024","format":"gallery","excerpt":"Role Global challenges require global actions to achieve Sustainable Goals while supporting a circular economy. Biomass is a renewable versatile resource that can be used to produce heat, power, transport fuels and products, however, it should be sustainable. In an effort to achieve the UK\u2019s net zero target the UK\u2026","rel":"","context":"In &quot;Analytical chemistry&quot;","block_context":{"text":"Analytical chemistry","link":"https:\/\/ernestohernandez.org\/?cat=101"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3921,"url":"https:\/\/ernestohernandez.org\/?p=3921","url_meta":{"origin":4764,"position":2},"title":"Biomass Characterization by SEM-EDX","author":"Ernesto Hernandez","date":"July 5, 2023","format":false,"excerpt":"Climate change concerns and the post COVID world need urgent solutions to develop sustainable societies with better energy, products and services 1. Biomass could help but its chemical properties must be known in faster ways2. Our work delivered a faster and reliable method for elemental analyses of biomass with Scanning\u2026","rel":"","context":"In &quot;Analytical chemistry&quot;","block_context":{"text":"Analytical chemistry","link":"https:\/\/ernestohernandez.org\/?cat=101"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4584,"url":"https:\/\/ernestohernandez.org\/?p=4584","url_meta":{"origin":4764,"position":3},"title":"BRIDGE | BioRenewables Inspired Development Geared by Elements","author":"Ernesto Hernandez","date":"July 26, 2024","format":false,"excerpt":"The UK government\u2019s Biomass Strategy 2023 aims to enhance biomass sustainability to help achieve net zero targets. The BRIDGE program supports these goals through SEM-EDX analysis for biomass characterization, fostering impactful research, sustainable manufacturing, and providing research-oriented teaching. This enhances student learning on real-world sustainability issues and critical thinking.","rel":"","context":"In &quot;Agriculture&quot;","block_context":{"text":"Agriculture","link":"https:\/\/ernestohernandez.org\/?cat=125"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":4975,"url":"https:\/\/ernestohernandez.org\/?p=4975","url_meta":{"origin":4764,"position":4},"title":"Microbially Induced Carbonate Precipitation (MICP): New Strains, New Perspectives","author":"Ernesto Hernandez","date":"November 22, 2024","format":false,"excerpt":"Allala F. 1 *, Mechri Sondes, Chehr\u00ee Ryma, Hadjari Ouiam, Hammdoud Ikhlas,Bouacem K. 1, 2, Jaouadi B. 3, Bouanane A. 1& Hernandez J. E.4 1 Laboratoire de Biologie Cellulaire et Mol\u00e9culaire (LBCM), Equipe de Microbiologie, Facult\u00e9 des Sciences Biologiques, Universit\u00e9 des Sciences et de la Technologie Houari Boumediene (USTHB), Alger,\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1290,"url":"https:\/\/ernestohernandez.org\/?p=1290","url_meta":{"origin":4764,"position":5},"title":"Job: QC Analyst, \u00a310.00 &#8211; \u00a312.00 per hour","author":"Ernesto Hernandez","date":"February 26, 2016","format":false,"excerpt":"Hi Ernesto, Apologies if this email is not relevant for you, I have sent it to multiple candidates in the local area. I have an\u00a0immediate, temporary\u00a0vacancy for a QC Analyst to join a successful company specialising in the contract manufacture of personal care and pharmaceutical products for a variety of\u2026","rel":"","context":"In &quot;Opportunities&quot;","block_context":{"text":"Opportunities","link":"https:\/\/ernestohernandez.org\/?cat=3"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"_links":{"self":[{"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/posts\/4764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4764"}],"version-history":[{"count":4,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/posts\/4764\/revisions"}],"predecessor-version":[{"id":4770,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/posts\/4764\/revisions\/4770"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/media\/4769"}],"wp:attachment":[{"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4764"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4764"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ernestohernandez.org\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}